Journal: Nucleic Acids Research
Article Title: The transcriptional regulatory network of the Escherichia coli MG1655 reference strain
doi: 10.1093/nar/gkag059
Figure Lengend Snippet: Overview of the PRECISE-MG1655 dataset. ( A ) Growth of single-protocol RNA-seq data for wild-type E. coli MG1655 present in the PRECISE, PRECISE-1K, and PRECISE-MG1655 datasets. ( B ) Visual representation of the composition of the condition-space of the PRECISE-MG1655 dataset based on select metadata categories [created in BioRender. Palsson, B. (2026) https://BioRender.com/w6f4azs ]. ( C ) Histogram of Pearson’s r correlation between gene expression (log 2 [TPM]) of all samples in the dataset, including both replicate (green bars) and non-replicate (blue bars) pairs. ( D ) Scatter plot of the first two principal components (PCs) in the dataset, colored by project ( n = 584). The metadata for each project is listed in .
Article Snippet: Sequencing libraries with an average insert length of ∼300 bp were created using a KAPA stranded RNA-Seq Kit (Kapa Biosystems KK8401) by following the manufacturer’s protocol.
Techniques: RNA Sequencing, Gene Expression